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Multi-parallel fermentation tank culture process refers to the parallel culture of multiple fermenters in the same system to improve production efficiency and resource utilization.
Here are some key points and precautions about this process:
Cultivation process：
1. Media preparation:
Ensure the accurate preparation of media components and use high-quality raw materials.
Sterilize the media to eliminate possible contaminants.
2. Inoculation:
Select suitable inoculation strains and maintain the activity of the strains.
Use aseptic techniques for inoculation to avoid cross contamination.
3. Optimization of fermentation conditions:
Monitor and adjust key parameters such as temperature, pH, dissolved oxygen, etc. to ensure a suitable fermentation environment.
Real-time tracking of changes in the fermentation process through online monitoring equipment.
4. Stirring and gas exchange:
Select appropriate stirring mode and speed according to the characteristics of the fermentation microorganisms.
Ensure sufficient gas exchange, especially in aerobic fermentation.
5. Fermentation time and harvest:
Determine the optimal fermentation time based on the generation of the target product.
Harvest the product in time to prevent excessive fermentation from causing decomposition of metabolites.
Precautions：
1. Aseptic operation:
Strictly follow aseptic operation procedures to avoid contamination during the culture process.
Check the sterility of equipment and consumables regularly.
2. Equipment maintenance:
Check the operation of fermenters and related equipment regularly to ensure their normal operation.
For multi-tank fermenters, pay attention to the coordination and cooperation between the tanks.
3. Data recording and analysis:
Record key data during the fermentation process, such as temperature, pH, dissolved oxygen, cell concentration, etc.
Analyze data regularly to optimize subsequent culture processes.
4. Safety protection:
Pay attention to safety during operation to prevent the leakage of chemicals and biological materials.
Wear appropriate personal protective equipment (PPE).
5. Environmental control:
Ensure that the environmental conditions (such as temperature and humidity) in the fermentation room are appropriate.
Clean the fermentation room and equipment regularly to prevent the growth of microorganisms.
Summary：
The culture process of multi-tank parallel fermenters is of great significance in improving production efficiency and achieving efficient use of resources. Through scientific and reasonable operation and strict management, the culture effect can be maximized, the cost can be reduced, and the product quality can be ensured.
